Metody identyfikacji ektomikoryz

Autorzy

• Dorota Hilszczańska Instytut Badawczy Leśnictwa, Zakład Ekologii Lasu, ul. Braci Leśnej 3, Sękocin Stary, 05-090 Raszyn
  Tel. +48 22 7150502, fax +48 22 227150504, e-mail: d.hilszczanska@ibles.waw.pl

Abstrakt

Identification of the fungi forming ectomycorrhiza is still a great challenge. Much of knowledge on fungal diversity resulted from field surveys of sporocarps. Two methods are currently being used to describe ECM fungal communities associated with root tips: molecular techniques and morphological classification. Morphological and anatomical descriptions of ectomycorrhizal roots have provided useful data for identifying the fungi below ground, but most species have not been described by this method. According to current knowledge morphotyping (based on morphological classification) can be very useful as the primary method of ectomycorrhizal classification, when used in conjunction with molecular techniques. Although molecular techniques based on restriction fragment length polimorphism- (RFLP) internal transcribed spacer (ITS) analysis of polymerase chain reaction (PCR) are effective tools to identificate ectomycorrhiza, but do have they limitations. For instance, many types of ectomycorrhiza remain unknown because no matching RFLP type is observed in available databases. It is seems that promising method for identifying ectomycorrhizas can be DNA-sequence analysis.

Słowa kluczowe

• identification
• morphotyping
• PCR-RFLP
• ectomycorrhiza