Mikrorozmnażanie wisienki stepowej (Cerasus fruticosa Pallas)

Micropropagation of sour cherry (Cerasus fruticosa Pallas)


  • Krystyna Szczygieł Instytut Badawczy Leśnictwa, Zakład Genetyki i Fizjologii Drzew Leśnych,
    05-090 Raszyn, Sękocin Stary, ul. Braci Leśnej 3, e-mail: szczygik@ibles.waw.pl
  • Tomasz Wojda Instytut Badawczy Leśnictwa, Zakład Hodowli Lasu i Genetyki Drzew Leśnych
    Sękocin Stary, ul. Braci Leśnej 3
    05–090 Raszyn


The aim of this study was to apply the micropropagation method via organogenesis to an endangered species, Cerasus fruticosa Pallas. This species is classified as rare and endangered according to the Polish Plants Red Book. The explants – winter-dormant apical and axillary buds from plants growing in Prof. S. Białobok’s Forest Arboretume in Forest District Syców – were used for culture initiation. Two basal media were applied for adventitious bud and shoot multiplication: WPM and MS supplemented with 0–2 mg•l-1 BAP, 0.1 mg•l-1 IBA, 10–30 g•l-1 sucrose and 200 mg•l-1 both glutamine and casein hydrolysate. The best results of adventitious shoot multiplication were achieved on WPM medium supplemented with 1 mg•l-1 BAP, 0.1 mg•l-1 IBA and 10–20 g•l-1 sucrose. The addition of 5 g•l-1 activated charcoal to WPM medium (without growth regulators) for one month improved adventitious shoot elongation. The half strength MS medium with 2 mg•l-1 of IBA was the most favourable for microshoot rooting. The acclimatized plants survived two winters and flowered. The micropragation applied method makes it possible to preserve ‘clean’ genotypes of this endangered species.

DOI 10.2478/v10111-010-0030-1
Source Leśne Prace Badawcze (Forest Research Papers), 2010, Vol. 71 (4): 351–355
Print ISSN 1732-9442
Online ISSN
Type of article
Original research article
Original title
Mikrorozmnażanie wisienki stepowej (Cerasus fruticosa Pallas)
Publisher Instytut Badawczy Leśnictwa, Sękocin Stary, Poland
Date December, 2010

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